Figure 4 | Scientific Reports

Figure 4

From: A single neonatal administration of Bisphenol A induces higher tumour weight associated to changes in tumour microenvironment in the adulthood

Figure 4

Evaluation of tumour Treg abundance by immunofluorescence. (a) Representative identification of T helper lymphocytes (white triangles △) and Treg (white arrowheads  ) by immunofluorescence staining of membranal CD4 (red) and nuclear Foxp3 (green); scale bar, 50 μm; original magnification, ×40. (b) Representative images of immunofluorescence staining of tumour samples. (c) Treg proportion expressed as the percentage of Foxp3+ cells of CD4+ cells; **p = 0.0031 vs Intact, p = 0.0070 vs Vehicle; data from 2 independent experiments are expressed as mean ± SD; n = 8.

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