Figure 4

Tregs expanded at 33 °C present remarkably anti-inflammatory Treg-specific phenotype. Expression of selected: Treg associated genes (Treg AG), transcription factors (TFs), FoxP3 expression controlling genes (FEC), genes encoding receptors associated with cell migration (Homing) and genes for multiple chemokines and cytokines (Chemokines and cytokines) in 14-day cultures of Tregs expanded at 33 °C (Tr33, n = 3) and 37 °C (Tr37, n = 3) are shown. Results for CD4⁺ Teffs (Teffs, n = 3) derived from the same donors and expanded for 14 days at 37 °C are shown as a control. Relative FPKM values were used for generation of the heatmap.