Table 1 The relationship between concentration of ISL and reaction rate*(n = 3).

Buffer (mL)

0.2

0.194

0.188

0.176

0.152

0.12

0.04

0

18 mmol/L ISL (mL)

0

0.006

0.012

0.024

0.048

0.08

0.16

0.2

18 mmol/L GL (mL)

0.2

0.2

0.2

0.2

0.2

0.2

0.2

0.2

β-D-glucuronidase solution (mL)

0.8

0.8

0.8

0.8

0.8

0.8

0.8

0.8

GAMG (mmol/L)

0.63 ± 0.02

0.79 ± 0.01

0.93 ± 0.02

1.2 ± 0.03

1.68 ± 0.04

1.71 ± 0.03

1.72 ± 0.01

1.71 ± 0.01

v (μmol/L·min)

2.63 ± 0.07

3.29 ± 0.04

3.86 ± 0.07

4.99 ± 0.1

7.01 ± 0.15

7.13 ± 0.13

7.16 ± 0.02

7.11 ± 0.03

ISL (mmol/L)

0

0.09

0.18

0.36

0.72

1.2

2.4

3