Figure 1 | Scientific Reports

Figure 1

From: Discovery and characterization of the feline miRNAome

Figure 1

Computational pipeline of data analysis. The figure illustrates the four steps of the computational pipeline employed to analyze the RNAseq data. Pre-Processing: raw data were processed by the SOLiD software Lifescope in order to obtain good quality mappable reads. miRNA detection: this step was carried out by applying miRDeep2 to the mappable reads. Post-processing: the output of miRDeep2 was further analyzed by BLAST against different databases in order to assess conservation of the predicted miRNAs and remove sequences matching other kinds of small RNAs. Data Analysis: this step consisted of the application of a series of ad-hoc scripts for the extraction of descriptive statistics. The tool IPA was used to perform the functional enrichment analysis of potential targets of the identified miRNAs, which were predicted by the software miRiam.

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