Figure 5
MGluR5 selective antagonist MPEP inhibits neutrophil transmigration through BBB in vitro. After mechanical scratch was performed on the monolayer of primary BMECs on transwells, EGFP-labeled neutrophils were added to the upper inserts of in vitro BBB models. In total, 50 μM MPEP was added 15 min prior to the mechanical scratch. The number of transmigrated cells were observed and calculated using a fluorescence microscope after 6 h. (A) Identification of EGFP-labeled neutrophils, which were obtained from the bone marrow of EGFP transgenic mice. Nuclei were stained with DAPI. (B) EGFP-labeled neutrophil transmigration through in vitro BBB models. “Negative” means the endothelial layer without scratch. “Positive” means scratches were performed almost everywhere on the confluent surface of the endothelial layer. The number of transmigrated cells is presented. Data are represented as the mean ± standard error of the mean (*P < 0.05).
