Figure 6
PKC signaling is involved in the regulation of mGluR5 on neutrophil trafficking. (A) In vitro, mechanical scratch (MS) was performed on mouse brain endothelial cells (bEnd.3, a BMEC cell line). In total, 50 μM MPEP was added 15 min prior to MS. Twenty-four h after MS, the mRNA expression of CXCL1, CXCL2, CCL2, CCL4 and CCL5 of bEnd.3 cells was examined via qRT-PCR. (B) PKC pathway, the main pathway downstream of mGluR5, was examined in a mechanical scratch (MS) model of bEnd.3 cells (a BMEC cell line) in vitro. In total, 1 μM PKC inhibitor GF109203X and 50 μM mGluR5 inhibitor MPEP were added 15 min prior to scratch. The mRNA expressions of CXCL1, CXCL2, CCL2, CCL4 and CCL5 of bEnd.3 cells were analyzed via qRT-PCR at 24 h post-scratch. (C) Western blot of p-PKC in bEnd.3 cells at 24 h post-MS and its quantitative analysis. Data are presented as the mean ± standard error of the mean (*P < 0.05, **P < 0.01, ***P < 0.001, #P < 0.05 relative to the other scratch groups, ##P < 0.01 relative to the other scratch groups, ###P < 0.001 relative to the other scratch groups, NS indicates no significant difference).
