Figure 2
Disrupted function of acidobacterial 16 S rRNA in E. coli is significantly restored following replacement of the 3′ minor domain sequence with that of E. coli 16 S rRNA. The doubling times (DTs) of E. coli KT105 strains carrying 16 S rRNA sequences of E. coli (Eco), Acidobacteria (NS11), and their chimeras (NS11-CntE, NS11-3MjE, and NS11-3MnE) are shown. The 16 S rRNAs of NS11-CntE, NS11-3MjE, and NS11-3MnE have chimeric sequences based on NS11 16 S rRNA, in which the central domain, 3′ major domain, and 3′ minor domain sequences were replaced with the corresponding sequences of Eco 16 S rRNA. For the detailed construction of these chimeric variants, see Supplementary Fig. 3. All the strains were grown in LB medium at 37 °C. The DTs were the average of four independent experiments (error bars, SD). The KT105 strain carrying the NS11-5E 16 S rRNA was excluded from the graph due to its lack of viability (see text).
