Figure 7 | Scientific Reports

Figure 7

From: Pseudo-acetylation of multiple sites on human Tau proteins alters Tau phosphorylation and microtubule binding, and ameliorates amyloid beta toxicity

Figure 7

hTau-4Q KI proteins displayed reduced microtubule-binding affinity. (a) In vivo microtubule-binding assay was performed on protein extracts from 2-day-old hTau-wt, hTau-4Q and hTau-4R KI fly heads. Taxol was used to induce microtubule polymerisation, and DMSO, the vehicle, was used as a control. S: supernatant, P: pellet. Western blots were cropped in this figure; full blots are shown in Supplementary Figure 19. (b) Quantification of the proportion of microtubule-bound hTau proteins in hTau-wt, hTau-4Q and hTau-4R KI lines, in Taxol conditions. Results are expressed relative to levels measured in hTau-wt KI flies (*p < 0.05, one-way ANOVA followed by Tukey’s post hoc test, n = 3/genotype).

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