Figure 2 | Scientific Reports

Figure 2

From: TCR–like antibodies mediate complement and antibody-dependent cellular cytotoxicity against Epstein-Barr virus–transformed B lymphoblastoid cells expressing different HLA-A*02 microvariants

Figure 2

EBV peptides EBNA1562-570, LMP1125-133 and LMP2A426-434 bind HLA-A*02 allelic variants. Peptide-MHC stability ELISA was performed to determine the binding of HLA-A*02:01-restricted EBV peptides to A*02:03, A*02:06 and A*02:07. Biotinylated MHC complexes with UV-cleavable cognate peptides were exchanged in the presence of the three respective EBV peptides. Exchanged complexes were added onto streptavidin-coated ELISA wells and β2m, which non-covalently interacts with the MHC class I, was subsequently detected as an indication of the structural integrity of the whole complex. Refer to Supplementary Figure S1 for a schematic illustration of the methodology.

Back to article page