Figure 5
Effect of LPS on HK-2 cells injury and expression of mPGES-2 in LPS-treated HK-2 cells. (A) The effects of treatment with LPS at different concentrations for 24 h on HK-2 cells viability (left panel) and LDH release (right panel). (B) Effects of 1000 ng/ml LPS treatment for different time on the viability of HK-2 cells (left) and LDH release (right). (C) Effects of LPS treatment for 12 h on expression of mPGES-2 mRNA (upper panel) and mPGES-2 protein (middle panel) in HK-2 cells. The lower panel shows the gray scale ratio of mPGES-2 protein to β-actin. (D) The expression of mPGES-2 mRNA (upper panel) and mPGES-2 protein (middle map) in HK-2 cells after different time of LPS treatment. The lower panel shows the gray scale ratio of mPGES-2 protein to β-actin. *P < 0.01, # P < 0.05versus the control group. Data are mean ± SD. (n = 3).
