Figure 7
Effects of mPGES-2 interference on autophagy and apoptosis of HK-2 cells treated with LPS. (A) Shown are the levels of mPGES-2 expression in HK-2 cells transfected with control siRNA and siRNA-mPGES-2 after treatment with 1000 ng/ml LPS for 12 h measured by qRT-PCR (left panel) and immunoblot analyses (middle panel). Right panel shows the ratios of mPGES-2/β-actin. (B) Shown are the immunoblot results of LC-3B and p62 (left panel) and the ratios of LC3B-II/LC3B-I and p62/β-actin (right panel) in HK-2 cells transfected with control siRNA and siRNA-mPGES-2 after treatment with 1000 ng/ml LPS for 12 h. (C) Shown are the immunofluorescence analyses of HK-2 cells in different groups treated with 1000 ng/mL LPS for 12 h (left panel, scale bar: 20 μm) and the average number of autophagosomes per cell measured in at least 30 cells (right panel). (D) Shown are the viabilities of HK-2 cells in different groups measured using CCK8 analysis. (E) Shown are the LDH cytotoxicity detection in different groups. (F) Shown are the apoptosis of HK-2 cells in different groups examined by flow cytometry. (G) Shown are the ratios of apoptotic cells in different groups (right panel). *P < 0.05 versus the control group. # P < 0.05 versus the LPS + control plasmid group. Data are mean ± SD. (n = 3).
