Figure 2
Whole-body glucose homeostasis and serum FGF21 levels of mice on MR, LR or control diet. Glucose tolerance was assessed after (a) 2 days on diet (data presented previously for MR vs. CON28) and (b) 5 weeks on diet by a GTT (2 g/kg) after a 5 h fast in mice fed MR (n = 10), LR (n = 10) or control (n = 9) diet. Significance was calculated by repeated measures two-way ANOVA. *Significantly different to MR and LR-fed mice (P < 0.05) for overall diet effect and interaction of diet with time. (c) Fasting serum insulin was measured after 6 weeks on diet, (d) fasting blood glucose was measured after 8 weeks on diet, (e) fasting serum FGF21 was measured after 6 weeks on diet, using tail vein blood samples taken after a 5 h fast in mice fed MR (n = 6–10), LR (n = 8–10) or control (n = 9) diet. Significance was calculated by two-tailed Student’s t-test. a,b,cMeans with letters that differ denote significance at level of P < 0.05. Data are represented as mean ± SEM. White circles/bars, control-fed mice; grey circles/bars, LR-fed mice; black circles/bars, MR-fed mice.
