Figure 1 | Scientific Reports

Figure 1

From: Raman spectroscopy uncovers biochemical tissue-related features of extracellular vesicles from mesenchymal stromal cells

Figure 1

EV characterisation by means of Western blotting and transmission electron microscopy. (A) Western blot analysis of extracellular vesicles-enriched fractions (EVs) produced by BM-MSC, ASCs and DFs using the indicated antibodies. Flotillin-1, CD63 and CD9 are positive markers for EVs and Calnexin is considered a negative one. Corresponding cell lysate (CL) was used as control and depicts the specificity of the three antibodies. Western blots were cropped to improve clarity. All bands within the range of the molecular markers were retained and processing of the film was applied equally across the entire image. (B–D) Representative transmission electron photomicrographs of ultracentrifuged EVs from BM-MSCs (B), ASCs (C) and DFs (D) conditioned medium. The TEM images were used for size measurements. Bars = 100 nm.

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