Table 1 Main Raman peak assignments from literature refs 65,66,67.

From: Raman spectroscopy uncovers biochemical tissue-related features of extracellular vesicles from mesenchymal stromal cells

Position (cm−1)

Component

520

Phosphatidyinositol

524

Phosphatidylserine

537

Cholesterol ester

540

Glucose-saccharide band

596

Phosphatidylinositol

701–703

Cholesterol ester

720–820

Nucleic acids

752–760

Tryptophan

840–860

Polysaccharide structure

941

Polysaccharide structure

995

C-O band of ribose

1003

Phenylalanine

1048

Glycogen

1054

C-O and C-N stretching of proteins

1060–1095

C-C vibrations of lipids and carbohydrates

PO2 stretching of nucleic acids

1120

C-O band of ribose; carotenoids

1127

C-N stretching of proteins; ceramides

1200–1300

Amide III

1230–1240 for β-sheets; 1260–1300 for α-helix

1298

Fatty acids

1336

CH3CH2 wagging mode of polynucleotide chain (purine bases)

1337

Tryptophan; Glycine backbone and Proline side chain

1357; 1361

Guanine

1360

Tryptophan

1420–1480

CH functional groups of nucleic acids, proteins and lipids

1520–38

Carotenoids

1555–1558

Tryptophan

1716–1740

C=O group

2853–2881

CH2 symmetric and asymmetric stretches of lipids

2910

CH 3 stretching vibrations

2940

CH and CH 2 in lipids and proteins

  1. The major divergent peaks are highlighted in bold characters.