Figure 5 | Scientific Reports

Figure 5

From: Both p62/SQSTM1-HDAC6-dependent autophagy and the aggresome pathway mediate CDK1 degradation in human breast cancer

Figure 5

CDK1 is recruited to aggresomes in response to proteasome inhibition. (A) NP40-soluble and -insoluble fractions from MCF7 cells treated with MG132 for the indicated times or etoposide (Eto) for 24 h were analyzed by immunoblot. Lanes are numbered from 1 to 10. (B, C) MCF7 cells treated or not with MG132 for 24 hours were analyzed by confocal microscopy. In the merge, CDK1 staining is shown in red and HDAC6 (B) or p62 (C) in green. Bars represent 5 μm.

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