Figure 6
Inhibition of SHIP2 activity does not reduce PA-induced ROS generation or protect cultured human podocytes from apoptosis. (A) PA increases SHIP2 activity of cultured human podocytes. Simultaneous treatment of podocytes with PA and SHIP2 inhibitor AS1949490 prevents PA-induced increase in SHIP2 activity. (B) PA induces ROS generation in cultured human podocytes as observed by DCFH-DA fluorescent probe assay. Hoechst 33342 was used for normalization. Treatment of human podocytes with SHIP2 inhibitor AS1949490 does not prevent PA-induced increase in ROS generation. (C) PA induces apoptosis in cultured human podocytes as visualized by annexin V staining and FACS analysis. Treatment of human podocytes with SHIP2 inhibitor AS1949490 does not prevent PA-induced increase in apoptosis. The experiments were performed three times with four (A), 32 (B) or three (C) replicates in each experiment. The bars show the mean expression in arbitrary units (error bars STDEV). **p < 0.01, ***p < 0.001, Student’s t-test.
