Figure 4 | Scientific Reports

Figure 4

From: Whole body and hematopoietic ADAM8 deficiency does not influence advanced atherosclerotic lesion development, despite its association with human plaque progression

Figure 4

Whole-body ADAM8 deficiency has no effect on advanced plaque size or morphology. (a,b) Plasma cholesterol (a) and triglyceride (b) levels of female whole-body wildtype (Adam8 +/+) and Adam8 −/− mice rendered hyperlipidemic by AAV8-PCSK9 gene transfer and subsequent western type diet (WTD) feeding, at start of WTD diet (0) and after 5 and 10 weeks (n = 16/14 mice, parametric Student’s t-test). (c,d) Quantification of the aortic root lesion area (c, n = 14/13 mice, nonparametric Mann-Whitney U test) and necrotic core area (d, n = 14/13 mice, nonparametric Mann-Whitney U test) of whole body Adam8 +/+ and Adam8 −/− mice after 10 weeks of WTD feeding. (e) Plaque stage classification (n = 42/39 atherosclerotic lesions in the aortic root) was scored (Fisher’s exact test). (f–h) Representative examples of (immuno)histochemical stainings for MAC-3+ macrophages (f, n = 14/13 mice, parametric Student’s t-test scale bar, 200 μm), Ly6G+ granulocytes (g, n = 14/13 mice, nonparametric Mann-Whitney U test scale bar, 50 μm) and Sirius Red stained collagen (h, n = 14/13 mice, nonparametric Mann-Whitney U test scale bar, 200 μm) with quantification.

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