Figure 2
From: Hedgehog signalling is required for cell survival in Drosophila wing pouch cells
Cell death caused by Hh signalling reduction is dpp-independent. (A) The dpp expression pattern in wild-type wing discs is revealed by the dpp-lacZ enhancer trap line (red). (B) dpp-lacZ is still present (red) when Smo (green) is suppressed within the dpp-Gal4 domain. (C) Omb (red) is still detectable when smo is inhibited in the dorsal compartment by the dorsal-specific driver ap-Gal4. The Hh target Ptc is apparently inhibited within the dorsal compartment (green). The dotted line indicates the boundary between the dorsal and ventral compartments. (D and F) In control experiments, dpp is expressed within the dpp-Gal4 domain (D) and the nub-Gal4 domain (F). No cell death occurs within the wing pouch regions (dotted regions) except for a patch of dead cells (red) in the presumptive hinge domains, which might be a side effect of overgrowth induced by excess Dpp. (E and G) The apoptosis (red) is still induced even when dpp is co-expressed with smo-RNAi in the dpp-Gal4 (E) and nub-Gal4 (G) domains. A specific marker for the anterior compartment, Ci, is revealed by anti-Ci staining (green), to show the midline of overgrown wing discs.
