Figure 3
From: Hedgehog signalling is required for cell survival in Drosophila wing pouch cells
The cell death induced by suppressing Hh signalling is JNK-independent. (A) Ectopic JNK signalling activity can be revealed by using a puc-lacZ. In a positive control experiment, puc-lacZ reporter (red) is activated when Dpp signalling is suppressed by expressing dad within the nub-Gal4 domain. (B,C) Suppression of Hh signalling by expressing smo-RNAi does not induce ectopic puc-lacZ (red). (D–I) Inhibition of JNK signalling by expressing a dominant negative form of bsk DN (E and G) or by suppressing an upstream effector (by expressing hep-RNAi) (I) can not rescue the apoptosis induced by expressing smo-RNAi. (D,F and H) The control experiments show no apoptosis when bsk DN or hep-RNAi is expressed alone. (J) Statistics for the apoptotic cell number per wing disc of each genotype mentioned above. ns stands for no significant difference.
