Figure 4
From: Hedgehog signalling is required for cell survival in Drosophila wing pouch cells
Cell death induced by the lack of Hh signalling activity requires the activation of the pro-apoptotic gene hid and the reduction of diap1. (A) In the control nub > GFP wing disc, there is no apparent hid-lacZ expression (red). (B) The pro-apoptotic gene hid (red) is activated in the wing pouch when smo-RNAi is expressed within the nub-Gal4 domain. (C,D) diap1-lacZ (red) is apparently reduced in the medial region of the nub > smo-RNAi wing discs (D) compared with the control (C). (E) Suppressing pro-apoptotic gene hid by expressing hid-RNAi efficiently suppresses the cell death compared with the control (Fig. 1F). (F) Co-expression of diap1 with smo-RNAi efficiently suppresses the cell death. (G) Suppressing initiator caspase activity by expressing dronc-RNAi largely suppresses the cell death induced by smo-RNAi expression. (H) Suppressing effector caspase activity by expressing drice-RNAi reduces the cell death induced by smo-RNAi expression. (I) Co-expression of P35 with smo-RNAi suppresses the cell death completely. (J) Statistical analysis for the apoptotic cell number per wing disc of each genotype mentioned above. Means ± SEM indicated *** are significantly different (pairwise comparisons performed using t-tests, p < 0.0001).
