Figure 6
From: Contribution of C1485T mutation in the HBx gene to human and murine hepatocarcinogenesis
Enhancement of GSK3β and Wnt activation in the liver of C1485T-HBx transgenic mice. Male control non-Tg mice, male HBx-WT Tg mice (Tg line 1), and male HBX-C1485T-Tg mice (Tg line 1) were treated with intraperitoneal injection of diethylnitrosamine (DEN, 100 mg/kg) and liver tissues were obtained 4 hours later. (a) Protein oxidation of liver lysate from acute DEN-treated mice was determined by oxyblot analysis kit. Lane 1, 2; control non-Tg mice, lane 3, 4; HBx wild type transgenic mice, lane 5, 6; HBx C1485T transgenic mice. (b) The signal intensity of each lane from oxyblot result (a) was determined by Image J software. The bar graph presented mean ± standard error (n = 3). (c) Immunoblot analysis for total and phosphorylated GSK-β and β-catenin with actin as a loading control; each number represents the ratio to the control samples on the lane 1. Lane 1, 2; control non-Tg mice, lane 3, 4; HBx wild type transgenic mice, lane 5, 6; HBx C1485T transgenic mice.
