Figure 2

Quality control examination of the tag-linkage method. (a) Two HBV clonal plasmids were used to examine processing errors. Fractions of reconstructed sequences are shown. More than 95% of errors result in false positive haplotypes that occur ≤0.1%, thus justifying 0.1% as our detection limit for minor viral haplotypes. (b) Successful detection of 5 different haplotypes is shown upon mixture of 5 plasmids in equal ratio. (c) Detection of 5 different haplotypes is shown upon mixing 5 plasmids in log ratio. The red dashed line represents the highest frequency of viral haplotypes detected in this sample which does not belong to the parental 5 plasmids, representing the level of noise. (d) Correlation of viral haplotypes of biological duplicates of mother sample of patient 1. (e) Correlation between clonal sequencing and tag-linkage sequencing. 40 random colonies were selected and their haplotype sequences were analyzed via both methods.