Figure 5 | Scientific Reports

Figure 5

From: TRIB3 inhibits proliferation and promotes osteogenesis in hBMSCs by regulating the ERK1/2 signaling pathway

Figure 5

Effects of TRIB3 knock-down on the activity of ERK. (A) Western blot analysis of ERK1/2 expression and activity in cells induced for 0, 3, 7, and 14 days. (B) Relative activity level of ERK protein quantified and plotted. (C) ERK activity measured by immunostaining for anti-p-ERK at days 0, 3, 7, and 14 of osteogenic differentiation. Scale: 40 μm. control shRNA: hBMSCs transfected with negative control-shRNA-Vector; TRIB3 shRNA: hBMSCs transfected with TRIB3-shRNA-Vector; TRIB3 shRNA + U0126: U0126 treatment of hBMSCs transfected with TRIB3-shRNA-Vector. *P < 0.05, **P < 0.01.

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