Figure 6

TRIB3 regulates the proliferation of hBMSCs by regulating ERK1/2 activity during osteogenic induction. (A) Cell proliferation at days 0, 3, 7 and 14 of osteogenic differentiation measured by EdU assay. Green: EdU dye; Blue: DAPI. Scale: 50 μm. (B) The proliferation rate from days 0 to 14 of osteogenic differentiation was measured using the CCK8 detection kit. control shRNA: hBMSCs transfected with negative control-shRNA-Vector; TRIB3 shRNA: hBMSCs transfected with TRIB3-shRNA-Vector; TRIB3 shRNA + U0126: U0126 treatment of hBMSCs transfected with TRIB3-shRNA-Vector. *p < 0.05 and **p < 0.01 used for comparison between TRIB3 shRNA and control shRNA; ^#p < 0.05, ^##p < 0.01, ^###p < 0.005 used for comparison between TRIB3 shRNA and TRIB3 shRNA + U0126.