Table 1 Molecular analysis of A/Chicken/Guangdong/J1/2017(H7N9) and A/Chicken/Guangdong/J2/2017(H7N9).

From: First genome report and analysis of chicken H7N9 influenza viruses with poly-basic amino acids insertion in the hemagglutinin cleavage site

Protein

Position

Mutation

Avian Strains

Human Strains

Biological feature altered by mutations

CK/J1

CK/J2

GD/17SF006

QY/GIRD1

TW/1

NS1

42

P-S

S

S

S

S

S

P42S increases virulence in mice29

92

D-E

D

D

D

D

D

Altered virulence in mice30

205

N-S

S

S

S

S

S

Altered antiviral response in host31

210

G-R

G

G

G

G

G

Altered antiviral response in host13

M2

31

S-N

N

N

N

N

N

S31N is known to confer resistance to amantadine and rimantadine32

NA

119

E-V

E

E

E

E

E

Oseltamivir resistance (N2 numbering)33

222

I-L

I

I

I

I

I

Oseltamivir resistance (N2 numbering)33

292

R-K

R

R

K

K

K

R292K is known to confer resistance to oseltamivir and zanamivir (N2 numbering)34

HA

CS

Basic aa insertion

PEVPKGKRTARGL

PEVPKGKRTARGL

PEVPKRKRTARGL

PEVPKRKRTARGL

PEVPKRKRTARGL

 

138

S-A

A

A

A

A

A

S138A increases virus binding to α-2,6-linked sialic acid receptor (human receptor) (H3 numbering)20

160

T-A

A

A

A

A

A

T160A increases binding to α-2,6-linked sialic acid receptor (human receptor) (H3 numbering)21

186

G-V

V

V

V

V

V

G186V increases binding to α-2,6-linked sialic acid receptor (human receptor) (H3 numbering)22

226

Q-L

Q

Q

Q

Q

Q

Q226L increases binding to α-2,6-linked sialic acid receptor (H3 numbering)25

228

G-S

G

G

G

G

G

Increased binding to human-type influenza receptor (H3 numbering)35

PA

100

V-A

A

A

V

V

A

Species-associated signature positions36

336

L-M

L

L

L

L

L

Increased polymerase activity in mice37

356

K-R

K

R

R

R

R

Species-associated signature positions38

409

S-N

N

N

N

N

N

Species-associated signature positions36

PB1

368

I-V

V

V

V

V

V

Increased transmission in ferrets18

PB1-F2

66

N-S

N

N

N

N

N

Induction of apoptosis39

PB2

627

E-K

E

E

K

K

K

 

701

D-N

D

D

D

D

D

D701N increases adaptation to mammals18

702

K-R

K

K

K

K

K

Species-associated signature positions40

  1. Abbreviation: CK/J1, CK/J2, GD/17SF006, QY/GIRD1, TW/1 represent for IAV(H7N9) strains A/Chicken/Guangdong/J1/2017(H7N9), A/Chicken/Guangdong/J2/2017(H7N9), A/Guangdong/17SF006/2017(H7N9), A/Guangdong/GIRD1/2017(H7N9) and A/Taiwan/01/2017(H7N9) respectively. aa short for amino acids; HA short for hemagglutinin; H3 short for hemagglutinin subtype 3; M2 short for matrix 2 protein; NA short for neuraminidase; N2 short for neuraminidase subtype 2; NS short for non-structural protein; PA short for polymerase acidic protein; PB short for polymerase basic protein; PB1-F2 short for polymerase basic protein 1 alternate reading frame 2. Underlined characters indicating the different amino acids among viruses.
Back to article page