Table 1 Dimethyl labelled and semi-tryptic peptides identified in MpnEf-Tu, MhpEf-Tu and SaEf-Tu.

From: Elongation factor Tu is a multifunctional and processed moonlighting protein

No.

ID

Peptide Sequence

Score

E-value

 

Dimethyl Labelled peptides from Mpn Ef-Tu

1

N1

K.58 ARGITINSAHVEYSSDKR75.H

41

3.00E−03

N2

R.60 GITINSAHVEYSSDKR75.H

94

1.20E−08

3

N3

R.131 MVVFLNK137.C

57

1.30E−03

5

N4

Q.242 EIEIVGLRPIR252.K

48

3.30E−03

N5

E.243 IEIVGLRPIR252.K

35

0.033

N6

I.244 EIVGLRPIR252.K

37

0.014

7

N7

K.305 FKAEIYALKKEEGGR319.H

110

9.40E−09

N8

K.307 AEIYALKKEEGGR319.H

69

2.60E−05

8

N9

R.320 HTGFLNGYRPQFYFR334.T

61

2.80E−05

N10

H.321 TGFLNGYRPQFYFR334.T

39

2.90E−03

N11

N.326 GYRPQFYFR334.T

43

1.70E−03

Semi-tryptic C-terminal Peptides from Mpn Ef-Tu

6

S1

R.253KAVVTGIEMFKKELD 267.S

47

4.50E−04

S2

R.253KAVVTGIEMFKKELDS 268.A

56

4.50E−05

S3

R.253KAVVTGIEMFKKELDSAMA 273.G

55

7.30E−05

S4

R.253KAVVTGIEMFKKELDSAMAG 272.D

95

4.50E−09

S5

R.253KAVVTGIEMFKKELDSAMAGDNA 275.G

96

3.30E−09

S6

R.253KAVVTGIEMFKKELDSAMAGDNAG 276.V

113

2.90E−09

S7

R.253KAVVTGIEMFKKELDSAMAGDNAGVLL 279.R

55

2.00E−05

7

S8

R.290GQVLAKPGSIKPHKKF 305.K

46

1.50E−04

S9

R.290GQVLAKPGSIKPHKKFKA 307.E

61

2.10E−06

S10

R.290GQVLAKPGSIKPHKKFKAE 308.I

27*

4.40E−03

S11

R.290GQVLAKPGSIKPHKKFKAEIY 310.A

38

2.40E−03

8

S12

R.290GQVLAKPGSIKPHKKFKAEIYALKKEEGG 318.R

85

7.00E−08

S13

R.290GQVLAKPGSIKPHKKFKAEIYALKKEEGGRH 320.T

24

8.20E−03

S14

R.290GQVLAKPGSIKPHKKFKAEIYALKKEEGGRHT 321.G

15*

0.048

S15

R.290GQVLAKPGSIKPHKKFKAEIYALKKEEGGRHTG 322.F

48

3.00E−04

S16

R.290GQVLAKPGSIKPHKKFKAEIYALKKEEGGRHTGFLN 325.G

25*

9.50E−03

S17

R.320HTGFLNG 326.Y

21*

0.058

 

Semi-tryptic N-terminal Peptides from Mpn Ef-Tu

1

S18

I.62 TINSAHVEYSSDKR75.H

37

4.60E−03

2

S19

D.111 SVMPQTREHILLAR124.Q

65

7.00E−05

4

S20

D.218 TMTITGR224.G

41

0.041

6

S21

L.267 DSAMAGDNAGVLLR280.G

73

2.40E−06

S22

D.268 SAMAGDNAGVLLR280.G

85

3.60E−06

S23

S.269 AMAGDNAGVLLR280.G

58

1.30E−03

S24

A.270 MAGDNAGVLLR280.G

53

7.20E−04

S25

M.271 AGDNAGVLLR280.G

75

1.90E−05

S26

A.272 GDNAGVLLR280.G

59

7.70E−04

S27

G.273 DNAGVLLR280.G

57

1.30E−03

S28

D.274 NAGVLLR280.G

42

0.031

8

S29

H.321 TGFLNGYRPQFYFR334.T

76

6.00E−06

S30

T.322 GFLNGYRPQFYFR334.T

47

3.00E−03

S31

G.323 FLNGYRPQFYFR334.T

77

3.00E−05

S32

L.325 NGYRPQFYFR334.T

62

5.30E−05

S33

N.326 GYRPQFYFR334.T

47

2.90E−04

S34

G.327 YRPQFYFR334.T

59

1.40E−03

9

S35

C.370 EKGSKFSIR378.E

66

1.30E−03

S36

C.370 EKGSKFSIREGGR382.T

35

6.60E−03

 

Dimethyl Labelled peptides from Mhp Ef-Tu

1

N1

M.2 AVVKTTGKKDFR14.S

84

5.70E−07

N2

A.3 VVKTTGKKDFR14.S

36

0.021

N3

V.4 VKTTGKKDFR14.S

34

0.019

2

N4

V.112 VAATDGPMPQTR123.E

74

1.70E−05

4

N5

A.316 AIYALKKEEGGR327.H

50.1

3.00E−05

 

Semi-tryptic N-terminal Peptides from Mhp Ef-Tu

3

S1

M.215 DKPFLMAVEDVFTITGR231.G

68

2.50E−05

 

Dimethyl Labelled peptides from Sa Ef-Tu

2

N1

D.101 GGILVVSAADGPMPQTR117.E

98

2.90E−05

N2

G.103 ILVVSAADGPMPQTR117.E

81

1.30E−03

N3

I.104 LVVSAADGPMPQTR117.E

104

5.90E−06

N4

L.105 VVSAADGPMPQTR117.E

91

1.10E−04

N5

V.107 SAADGPMPQTR117.E

69

9.90E−03

3

N6

N.137 KVDMVDDEELLELVEMEVR155.D

80

3.10E−03

N7

D.140 MVDDEELLELVEMEVR155.D

78

3.40E−03

4

N8

L.191 ELMEAVDTYIPTPER205.D

78

3.60E−03

N9

E.192 LMEAVDTYIPTPER205.D

78

3.20E−03

N10

L.193 MEAVDTYIPTPER205.D

72

2.80E−03

N11

M.194 EAVDTYIPTPER205.D

103

8.40E−06

N12

E.195 AVDTYIPTPER205.D

77

9.60E−04

7

N13

K.265 LLDYAEAGDNIGALLR280.G

98

4.10E−05

N14

L.267 DYAEAGDNIGALLR280.G

88

1.80E−04

N15

D.268 YAEAGDNIGALLR280.G

89

2.40E−04

N16

G.273 DNIGALLR280.G

67

1.80E−02

9

N17

R.335 TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTR374.F

86

7.70E−07

 

Semi-tryptic N-terminal Peptides from Sa Ef-Tu

8

S1

V.293 LAAPGSITPHTEFK306.A

106

3.20E−05

5

S2

M.214 PVEDVFSITGR224.G

80

0.010

1

S3

N.15 IGTIGHVDHGK28.T

80

1.10E−04

6

S4

F.263 RKLLDYAEAGDNIGALLR280.G

91

1.20E−03

  1. Identified peptides have a Mascot score >33 and an E-value <0.05 unless marked with a *. Peptides marked with a *implies the peptide score was <33 but still lies within major cleavage site. The exact site of cleavage is to the left of the amino acid that is bold and underlined for N-terminal cleavage fragments and to the right of C-terminal cleavage fragments. Amino acid numbers are written at the start and end of each peptide identified by LC-MS/MS.
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