Figure 5

Inflammatory cell-cell communication between large cholangiocytes. (a) Small and large cholangiocytes were incubated with PBS- or LPS-derived EVs isolated from small cholangiocytes for 48 hours. RT-PCR for proinflammatory cytokine expression (n = 6) and cell proliferation assay (n = 10) were performed. (b) Small and large cholangiocytes were incubated with EVs isolated from large cholangiocytes for 48 hours. RT-PCR (n = 6) and cell proliferation assay (n = 10) were performed.