Figure 2

Cav1 deficiency exacerbates retinal lesions in the acute ocular hypertension. IOP was elevated to 110 mm Hg, maintained for 1 hr and then released to the normal level. Retinae were collected 3d later. (A) No apoptosis was detected in Cav1 KO mice under intact condition. The eyeballs of 8-wk Cav1 KO and WT mice were harvest and cryosectioned for TUNEL staining. No apoptotic cells were detected. (B) TUNEL staining of retinal sections from WT and Cav1 KO mice. TUNEL⁺ cells were mainly located in the laminar layers. (C) Statistics of the TUNEL⁺ cell numbers from 3 independent experiments. More TUNEL⁺ cells were found in the Cav1 KO mice than in the WT mice. Scale bar, 50 μm.