Figure 5 | Scientific Reports

Figure 5

From: A chalcone derivative reactivates latent HIV-1 transcription through activating P-TEFb and promoting Tat-SEC interaction on viral promoter

Figure 5

Amt-87 promotes phosphorylation of CDK9 T-loop at Thr186. (A) Whole cell lysates derived from NH1 and J-Lat A2 cells, which were treated with (+) or without (−) Amt-87 for 24 hr, were analyzed by Western blotting to detect the levels of total CDK9 and CDK9 phosphorylated on Thr186 (pT186). The levels of pT186 were quantified by densitometry, normalized to those of total CDK9, and shown below the blots, with the level of pT186 in untreated cells (lanes 1 & 3) artificially set at 100%. (B) HeLa cells were pre-treated with flavopiridol (FLP) for 2 hr and then treated with Amt-87 for 24 hr. The levels of Pol II CTD phosphorylated on Ser2 (pSer2) and Spt5 phosphorylated on Thr775 (pT775) in whole cell lysates were examined by Western blotting and quantified by densitometry as in A and shown in below the blots.

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