Table 2 Viability and GFP production in J-Lat A2 cells treated with the indicated chalconoids.

From: A chalcone derivative reactivates latent HIV-1 transcription through activating P-TEFb and promoting Tat-SEC interaction on viral promoter

 

Compound

R″

% GFP(+) cells (μg/mL)

% Cell viability (μg/mL)

25

50

100

25

50

100

1a

4-CONHCH2CH2-3′-Thi

6.6 ± 0.7

9.2 ± 2.3

8.3 ± 1.6

67.1 ± 6.8

54.3 ± 5.4

41.8 ± 4.2

1b

4-CONHPh

3.1 ± 0.6

3.6 ± 1.2

3.0 ± 0.8

31.5 ± 4.4

15.7 ± 3.1

7.9 ± 2.2

1d

4-CONH(CH2)2Ph

13.7 ± 1.6

15.1 ± 2.4

15.7 ± 2.1

36.9 ± 3.9

35.0 ± 2.6

28.9 ± 3.7

1f

4-CONH(CH2)4Ph

2.5 ± 0.7

4.0 ± 1.3

7.2 ± 1.3

68.8 ± 3.1

65.2 ± 4.2

58.5 ± 4.5

1g

4-CONHCH2-1-Nap

10.3 ± 2.0

2.8 ± 1.0

1.8 ± 0.8

37.2 ± 6.2

27.9 ± 3.2

9.5 ± 1.5

1h

4-CONH-Ph-2′-OCH3

18.3 ± 1.3

22.5 ± 3.4

33.7 ± 3.6

49.3 ± 5.3

42.7 ± 4.0

12.3 ± 2.1

1k

4-CONH(CH2)5CH3

3.7 ± 1.2

3.9 ± 1.1

3.1 ± 1.0

67.6 ± 3.2

63.2 ± 4.2

53.3 ± 3.6

1l

4-CONH(CH2)7CH3

7.1 ± 1.7

8.4 ± 1.4

6.7 ± 1.5

24.7 ± 2.3

24.3 ± 2.7

19.5 ± 2.1

1o

3-CONHCH2CH2-3′-Thi

0.8 ± 0.1

3.8 ± 1.2

0.7 ± 0.1

36.4 ± 2.3

34.8 ± 2.7

37.3 ± 3.4

1p

3-CONH(CH2)2Ph

0.9 ± 0.1

2.1 ± 0.2

0.6 ± 0.1

42.0 ± 4.4

31.1 ± 2.0

31.2 ± 3.2

3c

4-MOM-5-Ad

0.7 ± 0.1

4.3 ± 1.1

13.3 ± 1.8

95.3 ± 3.5

92.5 ± 4.3

68.4 ± 4.2

3d (Amt-87)

2,4-diMOM-5-Ad

0.8 ± 0.1

5.9 ± 1.5

38.0 ± 4.2

96.3 ± 3.8

91.5 ± 3.4

72.9 ± 4.9

4c

4-OH-5-Ad

0.7 ± 0.1

3.8 ± 0.7

11.2 ± 2.0

86.4 ± 3.7

68.9 ± 6.3

45.4 ± 5.1

  1. J-Lat A2 cells were treated with the indicated compounds at 25, 50 and 100 μg/mL concentrations for 24 hr and the percentage of GFP(+) cells and cell viability were evaluated by FACS and Cell viability analysis, respectively. Data are presented as mean ± SD from at least three independent experiments that were performed in triplicates.
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