Figure 4
Immunohistochemical analysis of innervation and vascularization of healing skin. (a) Representative images of AEC+ chromagen βIII tubulin (dark red dots – white arrows) staining of the grafts. (b) Analysis of the total length of the graft re-innervated expressed as % shows a significant increase in PCL-RGD and PCL-bGE scaffolds compared to gauze control (asterisks). (c) Analysis of total number of nerve fibers crossing basal layer of epidermis shows a significant reduction from uninjured skin (letters) but no significant differences between scaffolds. Bar = 100μm. Data is presented as mean ± SEM for n = 3 for each group. ** represents p < 0.01. b represents p < 0.01 and c represents p < 0.05.
