Figure 6
ECM components produced by hSKPs. (a) Cell proliferation determined by trypan blue at 1, 3 and 7 days. The initial cell density at day 0 was 30,000 cells/gel as indicated by the dotted line. (b) DNA content at 3, 14 and 28 days of hSKPs culture measured by CyQuantTM showing the influence of RGD and gelatin on cell proliferation. The dotted line represents the DNA content immediately after seeding. (c) Glycosaminoglycan quantification of various nanofiber meshes at 3, 14 and 28 days determined by DMMB (d) GAG content normalized to total DNA content. # represents p < 0.05 which is statistically significant from day 14 and 28. (e) Total collagen content as measured by hydroxyproline calorimetric assay. (f) Collagen/DNA ratio. The data represents higher collagen and GAGs level on nanofiber scaffolds immobilized with RGD and composite with gelatin. Data is presented as mean ± SEM for n = 5 for each group. **** represents p = 0.0001, *** represents p < 0.001, ** represents p < 0.01 and * represents p < 0.05.
