Figure 8

Second harmonic generation images of collagen deposited on nanofiber scaffolds seeded with TdTomato⁺ hSKP. (a) Representative SHG images of scaffolds without cells and with cells at 2 and 4 weeks in culture. SHG is represented by white and TdT⁺ cells in red. (b) Mean intensity of the SHG signal shows a decrease between 2 and 4 weeks for the PCL-cGE scaffold and an increase between 2 and 4 weeks for the PCL-bGE scaffold (asterisks). A significant increase at 2 and 4 weeks for the PCL-cGE compared to gauze control was also noted (letters). (c) Mean intensity of the SHG signal normalized to control shows a significant difference between PCL and PCL-cGE at 2 weeks and between PCL and PCL-bGE at both 2 and 4 weeks (asterisks). Bar = 100 μm. Data is presented as mean ± SEM for n = 3 for each group. **** represents p < 0.0001 and * represents p < 0.05. b represents p < 0.01 and c represents p < 0.05.