Figure 2
From: Discovery of human-like L-asparaginases with potential clinical use by directed evolution
Colony formation of the BW2Δ strain is dependent on the ammonium product of the L-asparaginase reaction but not on the Asn KM of the enzyme. Colony formation at 37 °C after 72 h of the control strain BW2Δ pBAD and the L-asparaginase expressing strains: BW2Δ gpASNase1 (low Km enzyme) and BW2Δ pBAD_hASNase1 (high Km enzyme) on minimal medium M9 agar supplemented with increasing concentration of Asn (0, 0.2, 0.5 and 2 mM) and 0.02% of arabinose. Whereas expression of both L-asparaginases promotes colony formation, both the low and high Km enzymes do this with similar efficiency.
