Figure 3
From: Discovery of human-like L-asparaginases with potential clinical use by directed evolution
Colony formation of the BW5Δ strain is dependent on the Asp product of the L-asparaginase reaction and on the Asn Km of the enzyme. Colony formation at 37 °C after 72 h of the L-asparaginase expressing strains BW5Δ pBAD_gpASNase1 and BW5Δ pBAD_hASNase1 on minimal medium M9 supplemented with increasing concentrations of Asn (0, 0.2, 0.5 and 2 mM) and 0.02% arabinose. More and larger colonies are produced in the bacteria expressing the low KM gpASNases1 enzyme in an Asn concentration dependent manner.
