Figure 7
From: Discovery of human-like L-asparaginases with potential clinical use by directed evolution
All low KM chimera clones from the screen contained residues from gpASNase1 in regions #1 and #2. (A). Shown is a ribbon representation of the tetrameric gpASNase1 (PDB ID 4R8L), where each monomer is colored differently, the bound Asp reaction product is shown as green spheres, and regions #1 and #2 of protomer A colored in magenta. (B) Zoom on region #1. (C) Zoom on region #2. Note how region #1 is in close proximity to the active site of the same protomer, and region #2 is in close proximity to the active site of the adjacent protomer. Hence, both regions can play a role in the properties of the active site, thereby modulating the KM property.
