Figure 4
ASA or NaSA application cannot rescue the fertility of gme-2. (A) Real-time PCR analysis of the GME expression levels in WT, GME/gme-1 heterozygous and gme-2 homozygous plants. ACTIN2 was used as the internal control. Error bars represent the SE (n = 3). Asterisks represent Student’s t-test significance compared with wild type (**P < 0.01). (B) Real-time PCR analysis of GME in pollen grains at floral stage 13 from WT and gme-2 homozygous plants. (C) Total ascorbate contents in leaves from 5-week-old WT, GME/gme-1 and gme-2 plants. FW, fresh weight. Error bars represent the SE (n = 3). (D) Inflorescences from 6-week-old WT and gme-2 plants were treated with mock, 1 mM ASA or 1 mM NaSA for 10 days, and the derived siliques were imaged. (E) In vitro pollen germination of qrt/qrt and qrt/qrt GME/gme-1 treated with mock or 50 µM ASA. (F) Pollen germination rates in qrt/qrt and qrt/qrt GME/gme-1 treated with 0, 10, 50, 100, 500 or 1000 µM ASA, respectively. Error bars represent the SE (n = 3). (G) The maximum number of germinated pollen grains per tetrad of qrt/qrt and qrt/qrt GME/gme-1 in (E).
