Figure 1
From: Potential Antigens Involved in Delayed Xenograft Rejection in a Ggta1/Cmah Dko Pig-to-Monkey Model
Identification of primary RMEC and AEC from GGTA1/CMAH DKO pigs (A) Expression of CD31, von Willebrand factor(vWF), SLA class I (SLAI), and SLA class II DR (SLA II DR) on DKO RMEC and AEC was confirmed by flow cytometry. (the ‘controls’are isotype controls) (B) Expression of Gal and Neu5Gc on WT and DKO RMEC and AEC was confirmed by staining with BS-IB4 lectin and an anti-Neu5Gc monoclonal antibody, respectively. (C) The human serum IgG binding to the WT, GGTA1-KO, and DKO pigs RMEC and AEC respectively. (D) The human serum IgM binding to the WT, GGTA1-KO, and DKO pigs RMEC and AEC respectively.
