Figure 6

Effects of SCFAs and HDAC inhibitors on histone acetylation in ChIP assays. YAMC (A) and Caco-2 (B) cells were treated with DMSO, 10 nM TCDD alone or in combination with SCFAs and HDAC inhibitors for 24 hr, and whole cell lysates were analyzed by western blots. YAMC (C) and Caco-2 (D) cells were treated with DMSO, 10 nM TCDD and 5 mM butyrate alone or in combination for 4 hr, and ChIP assays were carried out as outlined in the Materials and Methods to detect interactions with the XRE-containing regions of the Cyp1a1 (mouse) and CYP1A1 (human) promoters.