Figure 1
From: The histone deacetylase SIRT6 blocks myostatin expression and development of muscle atrophy
Loss of SIRT6 leads to up-regulation of Myostatin in muscle cells. Mstn protein level (A,B) and mRNA expression (C) was determined in stable C2C12-scrambled (scr) and SIRT6-shRNA (T6KD) cells by immunoblotting or by qPCR respectively. For both techniques, β-actin expression served as a control. In the same cells, Atrogin1 (D), MyoD (E) and Myogenin (F) mRNA levels were determined by qPCR. (G) Stable C2C12-T6KD cells were infected either with empty (Null) or with SIRT6 over-expressing (T6OE) adenovirus, and then differentiated for 72 hours in differentiation medium (DM). DM-0 hour was used as an undifferentiated control (CN). MyoD protein expression was analyzed by immunoblotting (G) and quantified (H). All bar graphs represent mean ± SEM; n = 3 experiments; *p < 0.05, **p < 0.01.
