Figure 2
From: The histone deacetylase SIRT6 blocks myostatin expression and development of muscle atrophy
Effect of SIRT6 deficiency on cardiac expression of muscle-atrophy related genes. (A) Neonatal rat ventricular cardiomyocytes (cardiomyocytes) were infected with empty adv. (Null) or rat SIRT6-shRNA adenovirus (T6KD) to knockdown SIRT6, and expression of Mstn mRNA was determined by qPCR. (B) In the same group of cells, Mstn and Acvr2b expression was determined by immunoblotting. Actin was used as the loading control. (C) Quantitation of Mstn and Acvr2b expression in T6KD-cardiomyocytes, compared to Null control. (D,E) By qPCR, expression of follistatin and IGF1 mRNAs was measured in Null-adv.-infected and T6KD-cardiomyocytes. (F) Protein lysates from hearts of wild type (WT) or SIRT6-KO mice were immunoblotted with Mstn, Activin, angiotensinogen (AGT) and SIRT6 antibodies. Coomassie-stained blot was used as loading control. Results of three mouse hearts are shown for each group. (G) Quantitation of activin and AGT protein levels in WT and SIRT6-KO hearts. Values represent mean ± SEM for three independent experiments. *p < 0.05, **p < 0.01.
