Figure 4
From: The histone deacetylase SIRT6 blocks myostatin expression and development of muscle atrophy
Mstn released from SIRT6-deficient cardiomyocytes is functionally active. (A) Secreted Mstn was measured by ELISA in the condition-medium (CM) collected from cardiomyocytes infected with null or SIRT6-KD adenovirus. (B) CM harvested as in A was immunoblotted with actin antibody to demonstrate lack of cell lysis. Cell lysate (5 µg) was used to confirm efficacy of actin antibody and blot was stained with coomassie stain to visualize loading of CM. (C) Representative confocal images of differentiated C2C12 cells maintained in differentiation medium (DM) + CM from null or T6KD-adenovirus infected cardiomyocytes and immuno-stained with Myosin heavy chain (MF20) antibody (Green). Nuclei were stained with DAPI (Blue) and pictures were merged with DIC to display cell morphology. Scale bars are as indicated. (D) Quantitation of data acquired in experiments shown in panel C for myosin heavy chain (MyHC)-positive C2C12 cells. All bar graphs represent mean ± SEM for n = 3 experiments, *p < 0.05.
