Figure 6
From: The histone deacetylase SIRT6 blocks myostatin expression and development of muscle atrophy
SIRT6 over-expression mitigates atrophic effects of Mstn on muscles cells. (A) Phase-contrast images of proliferating C2C12 cells infected with null or SIRT6 over-expressing (T6OE) adenovirus and then treated for 24 hours with C26-condition-medium (CM). CM harvested from C26 colon cancer cells was diluted 1:1 with complete growth medium. Scale bars are as indicated. (B) Protein lysates from C2C12 cells differentiated for 3 days in DM, over-expressed with null or SIRT6 over-expressing (T6OE) adenovirus and then treated with TNF-α (20 ng/ml) or IFNγ (100 u/ml) or both as indicated for 48 hours were analyzed by immunoblotting with indicated antibodies. Protein lysates prepared from cells just before exposing them to DM is indicated as zero hour (0 hr). (C) Myofilament proteins extracted from C2C12 cells, treated as in panel B, were immunoblotted with MyHC (MF20) and Actin antibodies. (D) Protein lysates from stable C2C12-T6KD cells infected with null or SIRT6 over-expressing adenovirus and treated with TNF-α+IFNγ as mentioned above in panel B were immunoblotted with indicated antibodies.
