Table 2 Primers used for real-time PCR: m: mouse, r: rat.
From: The histone deacetylase SIRT6 blocks myostatin expression and development of muscle atrophy
Gene | Sequence (5′-3′) |
|---|---|
mMstn-F | CAGCCTGAATCCAACTTAGG |
mMstn-R | TCGCAGTCAAGCCCAAAGTC |
mMyogenin-F | GCTCAGCTCCCTCAACCAG |
mMyogenin-R | ATGTGAATGGGGAGTGGGGA |
mMyoD-F | GATGGCATGATGGATTACAGC |
mMyoD-R | GACTATGTCCTTTCTTTGGGG |
mAtrogin1-F | AACATGTGGGTGTATCGG |
mAtrogin1-R | TCTTGAGGGGAAAGTGAG |
mβ-Actin-F | TTCTTTGCAGCTCCTTCGTTGCCG |
mβ-Actin-R | TGGATGGCTACGTACATGGCTGGG |
rMstn-F | AGAGAGAGGCGAATGTGGAA |
rMstn-R | TCACTGCTGTCATCCCTCTG |
rMuRF1-F | TGTTCTGGTAGGTCGTTTCCG |
rMuRF1-R | ATGCCGGTCCATGATCACTT |
rFollistatin-F | AATGCCTACTGTGTGACCTGT |
rFollistatin-R | GGCTCATCCGACTTACTGT |
rIGF1-F | GGGAGACAACCTTGTCAAGCACCTA |
rIGF1-R | GAAATGAATGCCTGCTGAGGT |
rSIRT6-F | TTTATTGTTCCCGTGCGGCG |
rSIRT6-R | GGGTCGAATATCTCGGGCAG |
rβ-Actin-F | GACTTCGAGCAAGAGATGGCCACT |
rβ-Actin-R | AGCACTGTGTTGGCATAGAG |
