Figure 2 | Scientific Reports

Figure 2

From: Antibacterial and immunomodulatory activities of insect defensins-DLP2 and DLP4 against multidrug-resistant Staphylococcus aureus

Figure 2

Expression of DLP2 and DLP4 in P. pastoris X-33 in a 5-l fermentor. (A,B) Time curve of the concentration of secreted protein levels and cell wet weights during induction. (A) DLP2 expression; (B) DLP4 expression. Triplicate observations were made, and bars represent the standard error of the mean. (C,D) Tricine-SDS-PAGE analysis of the fermentation supernatants at different induced time. Lanes M: protein molecular weight marker (5 μl); others lanes: fermentation supernatants (10 μl) of DLP2 (C) and DLP4 (D) were taken at 24, 48, 72, 96 and 120 h, respectively. The full-length gels are presented in Supplementary Figure 6. (E,F) The inhibition zones of fermentation supernatant against S. aureus ATCC25923 and MRSA ATCC43300. Fermentation supernatants of DLP2 (12 μl) (E) and DLP4 (20 μl) (F) were taken at 0, 24, 48, 72, 96, and 120 h, respectively. The full-length inhibition zones are presented in Supplementary Figure 7.

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