Figure 3
Monitoring the inhibition of EV71 3Cpro activity by Rupintrivir using the i-3CS-GLuc2 biosensor. (A) Cell viability of HEK293T cells treated with various concentrations of Rupintrivir determined by CCK-8 assay. The results are presented as the means ± SD of triplicate measurements. (B) Detection of the cleavage of i-3CS-GLuc2 by EV71 3Cpro treated with or without Rupintrivir via Western blot analysis. (C) Quantification of the EV71 3Cpro activity treated with or without Rupintrivir by luciferase assay. The results are presented as the means ± SD of triplicate measurements (Student’s t test; ns, nonsignificant, ***p < 0.001).
