Figure 7
From: Identification of key regions and residues controlling Aβ folding and assembly
Secondary structure dynamics of WT and singly substituted variants of Aβ40. Forty μM Aβ40 was prepared in 10 mM sodium phosphate, pH 7.4, and incubated with shaking at 37 °C. Secondary structure dynamics were assessed using CD during 842 h of incubation. Panels are (A) WT, (B) D-L17, and (C1 and C2) D-N27. The data from panel C1 are plotted in panel C2 with a narrower molar ellipticity range to make spectral comparison easier. (D) Spectra of WT and substituted variants at the end of secondary structure changes. (E) Values of [θ]214 are plotted versus incubation time (h). Lines obtained from linear regression analysis of the data were used to determine assembly rates dθ/dt. Numbers adjacent to each line are the calculated rates in kdeg cm2 dmol−1/hour.
