Figure 5
Effects of ARIP2 overexpression on MyD88, TLR2, TLR4 and CD14 expressions in Raw264.7 cells. (A) TNFα and MyD88 mRNA expressions were examined by RT-PCR in Raw264.7 cells transfected with pcDNA3 empty plasmid (Lane 1) or pcDNA3-ARIP2 expressing plasmid (Lane 2). The graph represented the relative levels of mRNA in three separate experiments, and the mRNA levels of PC control group were adjusted to 100%. **P < 0.01, compared with PC group. Full-length gels are presented in Supplementary Figure 4. (B) Expression of TLR2, TLR4 and CD14 on macrophages was detected by flow cytometry. The graph showed the TLR2, TLR4 and CD14 expressions on Raw264.7 cells in three independent experiments. □, PC group; ■ ARIP2 group. **P < 0.01, compared with PC group.
