Figure 6
Inhibitory effects of in vivo ARIP2 overexpression on production of inflammatory cytokines of mouse peritoneal macrophages. (A) Expression of ARIP2 mRNA was detected by RT-PCR in mouse peritoneal macrophages transfected in vivo with pcDNA3 empty plasmid or pcDNA3-ARIP2 expressing plasmid (ARIP2). The graph represented the relative levels of mRNA in three separate experiments, and the mRNA levels of pcDNA3 control group were adjusted to 100%. **P < 0.01, compared with control group. Full-length gels are presented in Supplementary Figure 5. (B) Levels of IL-1β and TNFα were detected by ELISA in the supernatants of the cultured in vivo pcDNA3 (PC) and ARIP2 expressing plasmid-transfected mouse peritoneal macrophages, respectively, untreated (Control) or treated with LPS 500 ng/ml. **P < 0.01, compared with PC group.
