Table 1 Primer sequences were used in RT-PCR.
Gene | Primer | Sequence (5′-3′) | Fragment size(bp) | Tm(°C) |
|---|---|---|---|---|
GAPDH | F | GATTGTTGCCATCAACGACC | 371 | 56 |
R | GTGCAGGATGCATTGCTGAC | |||
ActRIIA | F | ATTGGCCAGCATCCATCTCTTG | 294 | 55 |
R | GCCACCATCATAGACTAGATTC | |||
Smad3 | F | CCAGCACACAATAACTTGGA | 574 | 58 |
R | AGACACACTGGAACAGCGGA | |||
ARIP2 | F | GTCAGCCGTATCAAAGAGGATG | 444 | 56 |
R | CTTGTGGCAATACTTCTCTGGTG | |||
IL-1β | F | ATGGCAACTGTTCCTGAACTC | 819 | 56 |
R | GCCCATACTTTAGGAAGACA | |||
TNFα | F | CATGAGCACAGAAAGCATGATCCG | 121 | 55 |
R | AAGCAGGAATGAGAAGAGGCTGAG | |||
MyD88 | F | ATGGTGGTGGTTGTTTCTGACGATT | 169 | 57 |
R | CGCATATAGTGATGAACCGCAGGAT |
